queC [2019-07-31 13:24:25]

synthesis of the modified ribonucleotide queuosine

locus: BSU_13720

pI: 5.15

mw: 24.38 kDa

protein length: 219 aa

gene length: 660 bp

function: tRNA modification

product: unknown

essential: no

synonyms: ykvJ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

Gene

Coordinates: 1,439,448 1,440,107

The protein

Protein family

• queC family (single member, according to UniProt)

Structure

• [PDB|3BL5] [Pubmed|18491386]

Expression and Regulation

Operons

• genes: [gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]-[gene|BA6DD5C9AB9BFF9759E9ECC46CE3DF4EB7E67F52|queD]-[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]-[gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]
  description: [Pubmed|14660578]

  regulatory mechanism

  • [regulon|preQ1 riboswitch|preQ1 riboswitch]: antitermination, in the absence of queuosine [Pubmed|19285444], in [regulon|preQ1 riboswitch|preQ1 riboswitch]

  regulation

  • repressed in the presence of queuosine ([SW|preQ1 riboswitch]) [Pubmed|19285444]

Biological materials

Mutant

• MGNA-A789 (ykvJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/789 NBRP B. subtilis, Japan]
• BKE13720 ([gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTCCTCTCCTTCTCC, downstream forward: _UP4_GAATATATGGTGATGAAAGG
• BKK13720 ([gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTCCTCTCCTTCTCC, downstream forward: _UP4_GAATATATGGTGATGAAAGG

References

• 18491386,14660578,17384645,19285444,19354300,24003028,21410253,21375305,24663240